Characterization of Weak Protein Domain Structure by Spin-Label Distance Distributions

Heterogeneity of protein substates visualized by spin-label EPR.

The energy landscape of proteins is characterized by a hierarchy of substates, which give rise to conformational heterogeneity at low temperatures. In multiply spin-labeled membranous Na,K-ATPase, this heterogeneous population of conformations is manifest by strong inhomogeneous broadening of the electron paramagnetic resonance (EPR) line shapes and nonexponential spin-echo decays, which underg...

The spatial effect of protein deuteration on nitroxide spin-label relaxation: Implications for EPR distance measurement

Pulsed electron-electron double resonance (PELDOR) coupled with site-directed spin labeling is a powerful technique for the elucidation of protein or nucleic acid, macromolecular structure and interactions. The intrinsic high sensitivity of electron paramagnetic resonance enables measurement on small quantities of bio-macromolecules, however short relaxation times impose a limit on the sensitiv...

Characterization of Protein Induced Fluorescence Enhancement (pife): a Label-free Protein Assay with Short Distance Sensitivity By

Dynamic molecular structure of DPPC-DLPC-cholesterol ternary lipid system by spin-label electron spin resonance.

The hydrated ternary lamellar lipid mixture of dipalmitoyl-PC/dilauroyl-PC/cholesterol (DPPC/DLPC/Chol) has been studied by electron spin resonance (ESR) to reveal the dynamic structure on a molecular level of the different phases that exist and coexist over virtually the full range of composition. The spectra for more than 100 different compositions at room temperature were analyzed by nonline...

A weak approximation for the Extrema's distributions of Levy processes

‎Suppose that $X_{t}$ is a one-dimensional and real-valued L'evy‎ ‎process started from $X_0=0$‎, ‎which ({bf 1}) its nonnegative‎ ‎jumps measure $nu$ satisfying $int_{Bbb‎ ‎R}min{1,x^2}nu(dx)